Part:BBa_K4345000

NarX fused to mNeonGreen with a rigid linker

Three proteins constitute a two-component nitrate sensing system in Escherichia coli: NarX, NarL and NarQ. Together they are responsible for the expression of anaerobic respiratory genes. NarX and NarQ are histidine kinases that independently detect the presence of nitrate an transmit the signal to NarL. After autophosphorylation and thus dimerization of NarX or NarQ, NarL is phosphorylated. This enables the activated NarL to bind DNA and induce expression of specific genes. Both NarX and narQ contain two conserved histidine residues that correspond to the autophosphorylation sites of other, homologous, sensor-transmitter proteins (Cavicchioli et al., 1995). Cheung & Hendrickson (2009) elucidated the structure of the histidine kinase in the apo- and holo-state to be a four-helix bundle. To follow the expression of NarX, it is fused to mNeonGreen with a rigid linker.

This part is composed of BBa_K4345007 (NarX), BBa_K4345002 (rigid linker) and BBa_K4345009 (mNeonGreen).

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 659
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 659
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 260
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 659
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 659
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 2236
  

Usage and Biology

This particular narX protein was derived from E. coli.

Image obtained from Cheung & Hendrickson, 2009

References

Cavicchioli, R., Schröder, I., Schröder, S., Constanti, M., & Gunsalus, R. P. (1995). The NarX and NarQ Sensor-Transmitter Proteins of Escherichia coli Each Require Two Conserved Histidines for Nitrate-Dependent Signal Transduction to NarL. JOURNAL OF BACTERIOLOGY, 177(9), 2416–2424.

Cheung, J., & Hendrickson, W. A. (2009). Structural Analysis of Ligand Stimulation of the Histidine Kinase NarX. Structure, 17(2), 190–201. https://doi.org/10.1016/J.STR.2008.12.013

narX sensor histidine kinase NarX [ Escherichia coli str. K-12 substr. MG1655 ]. (2022, September 22). National Library of Medicine - National Center for Biotechnology Information. https://www.ncbi.nlm.nih.gov/gene/945788